The Rosalind Franklin Institute is welcoming Prof. Rainer Kaufmann on Thursday 7 November from 10:30.

To watch the seminar online, please sign up via the link below.

RAL site pass holders are welcome to attend in person in the Franklin’s first floor Hub. To join in person, please arrive in the R113 Franklin foyer at least 5 minutes before the start of the seminar and a member of the team will let you into the building.

Title:

Development of a novel setup for cryo super-resolution fluorescence microscopy in the context of cryo-ET

Abstract:

Super-resolution methods present a true fame changer for the field of correlative light and electron microscopy (CLEM). They allow bridging the big resolution gap between conventional fluorescence microscopy (FM) and electron microscopy (EM). However, super-resolution FM under cryo-conditions for correlation with cryo electron tomography (cryo-ET) is still at an early and experimental stage. To achieve the aim of using cryo-FM to localise and identify individual proteins in cryo-ET, the development of cryo-ET-compatible super-resolution cryo-FM is essential.

In my talk, I will focus on how cryo single molecule photo-physics determines super-resolution cryo-FM, and why novel concepts regarding the design of super-resolution cryo-FM setups play an important role, not only for imaging, but also to gain a better understanding of the altered photo-physics under cryo-conditions.

Biography:

Rainer Kaufmann gained his PhD in Physics from the University of Heidelberg developing single molecule based super-resolution fluorescence microscopy. Afterwards he spent 6 years as a postdoc at the University of Oxford, with a focus on combining super-resolution fluorescence microscopy with electron microscopy. He is one of the pioneers in the field of cryo super-resolution fluorescence microscopy.

Since 2017, he is a PI at the Centre for Structural Systems Biology (CSSB) in Hamburg and the Department of Physics at the University of Hamburg.